Catalog #: 7955-IL
15 Citations 3 Reviews Datasheet / COA / SDS
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All IL-17/IL-17A Proteins and Enzymes
Product Details
Citations (15)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas
Recombinant Human IL-17A (Human Cell-expressed) Protein Summary
Product Specifications
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is0.12-1.2ng/mL. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED50 for this effect is 1.5-7.5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155
Accession #
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked hom*odimer
Predicted Molecular Mass
15.1 kDa (monomer)
SDS-PAGE
15-23 kDa, reducing conditions
28-38 kDa, non-reducing conditions
Product Datasheets
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7955-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
7955-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
Bioactivity View Larger
Recombinant Human IL-17 (Catalog # 7955-IL) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.12‑1.2 ng/mL.
Reconstitution Calculator
Reconstitution Calculator
Background: IL-17/IL-17A
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1,2). Mature human IL‑17A shares 60%amino acid sequence identity with mouse and rat IL‑17A (3,4). IL‑17A is secreted by Th17 cells, gamma /δTcells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked hom*odimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17RA in complex with IL‑17RC or IL‑17RD (7,8). Both IL‑17RA and IL‑17RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (16, 17).
- Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
- Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
- Yao, Z. et al. (1995) J. Immunol. 155:5483.
- Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
- Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
- Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
- Wright, J.F. et al. (2008) J. Immunol. 181:2799.
- Rong, Z. et al. (2009) Cell Res. 19:208.
- Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
- Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
- Liang, S.C. et al. (2007) J. Immunol. 179:7791.
- O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
- Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
- Hsu, H. et al. (2008) Nat. Immunol. 9:166.
- Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
- Wang, L. et al. (2009) J. Exp. Med. 206:1457.
- Kryczek, I. et al. (2009) Blood 114:357.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
Related Research Areas
- B Cell Chemotaxis, Migration, and Adhesion
- Cytokines and Receptors in Tfh Cell Differentiation and Function
- gamma delta T Cells
- Helper T (Th) Cells
- IL-17 Family
- LTi Cells
- Macrophage Activation Markers
- Microglia Activation Markers
- Molecules Secreted by VSMC
- Multiple Sclerosis and Myelin Related Molecules
- Natural Killer T (NKT) Cells
- NCR-ILC3
- Neuroinflammatory Factors
- Search Cytokine and Growth Factor Products
- T Cell Cytokine Signaling
- Th17 Cells
Citations for Recombinant Human IL-17A (Human Cell-expressed) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15 Citations: Showing 1 - 10
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- TNF-? and Poly(I:C) induction of A20 and activation of NF-?B signaling are independent of ABCF1 in human airway epithelial cells
Authors: Cao, QT;Ishak, M;Shpilman, I;Hirota, JA;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease
Authors: P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell
Cell Reports, 2022-09-27;40(13):111439.
Species: Human
Sample Types: Organoid
Applications: Bioassay - Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L
Authors: G Margetts, S Kleidonas, NS Zaibi, MS Zaibi, KD Edwards
BMC complementary medicine and therapies, 2022-05-12;22(1):131.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway
Authors: EM Byrne, M Llorián-Sa, M Tang, A Margariti, M Chen, H Xu
Biomedicines, 2021-07-16;9(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - Characterization of the interleukin-17 effect on articular cartilage in a translational model: an explorative study
Authors: D Sinkeviciu, A Aspberg, Y He, AC Bay-Jensen, P Önnerfjord
BMC Rheumatol, 2020-05-12;4(0):30.
Species: Bovine
Sample Types: Whole Tissue
Applications: Bioassay - IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network
Authors: L Sun, M Girnary, L Wang, Y Jiao, E Zeng, K Mercer, J Zhang, JT Marchesan, N Yu, K Moss, YL Lei, S Offenbache, S Zhang
J. Immunol., 2020-03-13;204(8):2177-2191.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - IL-36? regulates mediators of tissue homeostasis in epithelial cells
Authors: JE Heath, GM Scholz, PD Veith, EC Reynolds
Cytokine, 2019-03-08;119(0):24-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - IFN-? and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection
Authors: S Muntaka, Y Almuhanna, D Jackson, S Singh, A Afryie-Asa, M Cámara, L Martínez-P
Infect. Immun., 2019-01-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis
Authors: J Chorachoo, S Lambert, T Furnholm, L Roberts, L Reingold, S Auepemkiat, SP Voravuthik, A Johnston
PLoS ONE, 2018-10-15;13(10):e0205340.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay - Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632
Authors: ED Anderson, I Sastalla, NJ Earland, M Mahnaz, IN Moore, F Otaizo-Car, TG Myers, CA Myles, SK Datta, IA Myles
PLoS ONE, 2018-09-12;13(9):e0198862.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - Electrophilic properties of itaconate and derivatives regulate the�I?B?-ATF3 inflammatory axis
Authors: M Bambouskov, L Gorvel, V Lampropoul, A Sergushich, E Loginichev, K Johnson, D Korenfeld, ME Mathyer, H Kim, LH Huang, D Duncan, H Bregman, A Keskin, A Santeford, RS Apte, R Sehgal, B Johnson, GK Amarasingh, MP Soares, T Satoh, S Akira, T Hai, C de Guzman, K Auclair, TP Roddy, SA Biller, M Jovanovic, E Klechevsky, KM Stewart, GJ Randolph, MN Artyomov
Nature, 2018-04-18;556(7702):501-504.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vagin*l Th17 and regulatory Tr1 responses
Authors: BA Stanfield, PJF Rider, J Caskey, F Del Piero, KG Kousoulas
Vaccine, 2018-04-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture - Population snapshots predict early haematopoietic and erythroid hierarchies
Authors: BK Tusi, SL Wolock, C Weinreb, Y Hwang, D Hidalgo, R Zilionis, A Waisman, JR Huh, AM Klein, M Socolovsky
Nature, 2018-02-21;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay - RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature
Authors: WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson
Front Immunol, 2018-01-29;9(0):80.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture - Impaired Tight Junctions in Atopic Dermatitis Skin and in a Skin-Equivalent Model Treated with Interleukin-17
PLoS ONE, 2016-09-02;11(9):e0161759.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why?
Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.
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Reviews for Recombinant Human IL-17A (Human Cell-expressed) Protein
Average Rating: 4.3 (Based on 3 Reviews)
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Recombinant Human IL-17A (Human Cell-expressed) Protein
By Anonymous on 02/24/2021
Application: In vitro bioactivity in cell culture
Recombinant Human IL-17A (Human Cell-expressed) Protein
By Rachel Levantovsky on 11/18/2020
Application: In vitro bioactivity in cell culture
Recombinant Human IL-17A (Human Cell-expressed) Protein
By Anonymous on 06/04/2019
Application: CellProlif
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Associated Pathways
- IL-17 Family Signaling Pathways
- Innate Lymphoid Cells Differentiation
- Th17 Differentiation
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